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The most direct way to study a proposed lipolytic agent is in isolated fat cells or adipose explants, measuring glycerol and free-fatty-acid release into the medium, cAMP accumulation, and the phosphorylation state of HSL
General MTase inhibitors SAH and sinefungin that target the SAM binding site exhibited an IC 50 value of 26.3 4.4 M and 3.9 0.3 M, respectively
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10.1016/j.jpba.2014.07.026 [DOI] [PubMed] [Google Scholar] Jordan V
