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FFPE tissue preparation for spatial proteomics Tissue samples were collected immediately after excision, cut into sections no thicker than 45 mm, and fixed in 10% neutral-buffered formalin (NBF) at room temperature for 24 h to preserve morphology and prevent autolysis and specimens were processed through a graded ethanol series for dehydration (70% ethanol for 1 h, 95% ethanol for 2 1 h, and 100% ethanol for 2 1 h) followed by clearing in xylene

Archived from the original on 29 September 2015

Bacterial cells cannot produce nucleic acids like DNA or RNA without folate

Adequate levels of vitamin B12 are also required for a healthy nervous system, pregnancy, and the ability to produce DNA

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