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coli using glutathione-sepharose affinity columns and confirmed their relative purity and mass by SDS-PAGE and Coomassie staining (Figure 1B) before subjecting each protein fragment to in vitro kinase assays with LRRK2 (Figures 1CG)

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Pfeiffer, B., Kinnealey, M., Reed, C., and Herzberg, G., American Journal of Occupational Therapy , 2005, vol

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