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The plate was incubated for further 30 min at 37 C without CO 2 for equilibration and then inserted into a Seahorse XFe96 Analyzer (Agilent Technologies) loaded with a hydrated cartridge, for measurement

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Studies comparing the consumption effects of whole spinach versus chopped spinach have shown an increased folate bioavailability, as traduced by increased plasma levels, upon disruption of the spinach matrix [39,64]