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The amplicon was cloned into pGFP_glmS 67 digested with BglI, blunted using Klenow and re-digested with KpnI

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At the genetic level, it has been observed that the expression of Gln metabolism-related genes in tumor cells is significantly higher than that in CD8+ T cells, underscoring the metabolic advantage held by tumor cells (16)

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The immature neonatal GALT is characterized by the production of higher levels of pro-inflammatory cytokines (137, 138), whereas anti-inflammatory capacities are diminished (139)

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When this ratio flips, it is often a sign that the body is dealing with more oxidative stress than it can handle

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Another factor that affects the efficacy of radiation therapy is the relative levels of oxygen in the tumor

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Digests were then labeled by TMT 16-plex reagent following the SL-TMT protocol (64)

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