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HKA (cell line derived from a keratoacanthoma of human skin), Mewo, and G361 (human melanoma cell lines) cells were procured from the Japanese Collection of Research Bioresources (JCRB, Osaka, Japan) and maintained in high-glucose DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Thermo Fisher Scientific)

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PMID 1356504

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Quick math example A 10 mg vial mixed with 2 mL gives 5 mg/mL

Never overtighten your mask to prevent leaks, as this can cause discomfort, facial marks, and pressure sores