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To gain further insights into the functional implications of the DEPs, functional enrichment analysis was performed on a subset of 142 proteins using ToppGene ( Metascape ( and Revigo ( The overlap and relationships among the DEPs were visualized using a Venn diagram generated with InteractiVenn ( Furthermore, the top five significantly enriched Gene Ontology (GO) terms from the ranked list of DEPs were analyzed
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The eluted proteins were buffer-exchanged to DPBS and concentrated using Amicon Ultra-15 Centrifugation Filter Units (Millipore) with a 30 kDa cutoff by centrifugation at (290400) g at 4 C
