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M2 macrophages support tumor growth and metastasis by secreting immunosuppressive cytokines, such as IL-10 and TGF-, and promoting angiogenesis and stromal remodeling

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Measurements of chlorophyll content and carotenoid content Leaf tissues (0.5 g) supernatant was extracted with 80% v/v acetone and absorbance was measured with a UVvisible spectrophotometer at 663, 645, and 470 nm

The tissues were embedded in OCT compound (Sakura Finetek Japan, Tokyo, Japan), frozen at -80C, and sectioned into 7-m thicknesses in a cryostat on HistoBond adhesive microscope slides (Marienfeld-Superior, Lauda-Knigshofen, Germany)