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The compensatory upregulation of SYK in SCs thus represents a crucial mechanism to maintain this glycolytic shift and mitigate mitochondrial dysfunction beyond the initial peak, thereby supporting the prolonged energetic and biosynthetic demands of regeneration

Western blots The total amounts of hippocampal proteins (20 g) from three mice of each group were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (cat# IPVH00010, Merck millipore, USA)

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These reversible reactions are catalysed by a series of enzymes, including ribulose-5-phosphate epimerase, ribose-5-phosphate isomerase, transaldolase (TALDO) and the thiamine-dependent enzyme transketolase (TK

The overall effect is a self-limiting peel, that is more predictable to administer, for maximum results with limited unwanted side-effects

The residues of the key 6 YGS 8 motif within the GCT fold are located in loop 1, while the unique dimerization loop 2 is flipped away from the enzymes core domain (Nguyen et al., 2020)