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The tissue samples were homogenized in PBS (pH 7.4) containing 0.1 mmol/L butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, United States) and sonicated for 30 s in an ice bath (Ultrasonic bath, Branson, United States)

Over time, connective tissue loses efficiency in collagen synthesis, matrix organization and antioxidant capacity, leading to fine lines, uneven texture, lower hair density and more visible scars

Measurement of entrapment efficiency The entrapment efficiency of DETA NONOate within NPs was confirmed using a previously reported method

As we saw in Figures 1-4 , cyclic pressure variations often have greater magnitude and frequency than can be reasonably determined before the system being placed into service

Chlorinity, conductivity and density of seawater