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Without context, it can be difficult to know what is reasonable, what drives the cost and which option actually fits your goals

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Even retired or recreational athletes should understand the rules of their sport

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Common issues include unrecognised upper or lower limits of experimental methods, inadvertent inclusion of competing ligands ( eg buffers, salts), presence of metal-binding protein tags, poor pH control, the formation of adducts, non-equilibrium assays, oxidation of metal or ligand, contaminating metals, and misinterpretation of experimental data

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