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When the redox balance is disrupted, excessive production of free-radicals start to cause significant damage as the body is left unable to counteract the overproduction of these oxidant agents

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Cell Commun Signal 23 , 160 (2025)

[Updated 2023 Jul 24]

Then, 50 L of the resulting mixture was incubated at 22C-24C for 5 min, and the absorbance was measured at regular intervals of 5 min for up to 1 h at 450 nm

External factors, including UV exposure, oxidative stress, pollution, and lifestyle choices, can accelerate this process by weakening the skins barrier and increasing the breakdown of collagen and elastin