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As a result, clinical trials have confirmed that reintroduction of chloroquine is highly efficacious [32, 33]

This enzyme requires the FAD coenzyme form of riboflavin for this reduction reaction, thus, emphasizing the role of riboflavin in the formation of reduced, active, glutathione ( Second, it has been shown that the oxidation of dihydroriboflavin, the reduced riboflavin, forming oxidized riboflavin can deactivate lipid peroxides, emphasizing the independent antioxidant property of riboflavin ( Third, riboflavin effects on antioxidant enzymes activities, including superoxide dismutase (SOD) ( Important to note, UV-B irradiation, the atmospherically predominant UV radiation, has been shown to reduce the neuroprotective effects of riboflavin both in vitro and in vivo

Moreover, during parasitic diseases, phagocytic cells produce a high ROS and reactive nitrogen species (RNS) that contribute to the pathogenesis [37]

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Cell lysate was centrifuged and the soluble roGFP proteins purified via a Ni 2+ loaded HiTrap Chelating HP Column (GE Healthcare, www.gelifesciences.com)

Relevant to the above cases, High dose FA can improve BH4 recycling due to restoration of dihdrofolate reductase expression [35]