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Total RNA, DNA, and protein were extracted from the cells using an AllPrep DNA/RNA/Protein Mini Kit according to the manufacturers instructions (Qiagen N.V., Venlo, The Netherlands)

Such damage can be further exacerbated by neutrophil activation at the injury site

This ATF4-mediated induction is often triggered by the GCN2-eIF2 pathway when uncharged tRNAs accumulate (a sign of amino acid deprivation) [23]

Endocannabinoid Receptors in the CNS: Potential Drug Targets for the Prevention and Treatment of Neurologic and Psychiatric Disorders

Briefly, cotton stoppered shake-flasks were used with a working mass of 50 g containing water insoluble solids (WIS) content of 5 or 10% (w/w) steam-pretreated spruce (SEKAB E-Technology rnskldsvik, Sweden)

doi: 10.1016/j.neuron.2016.06.033 22 YuYZengZXieDChenRShaYHuangSet al