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Finally, they were resuspended in 1 mL of 100 mM Tris solution and the total proteins present in the final samples were quantified using QuantiPro BCA assay (Sigma Aldrich)

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High oxidative stress and the defective antioxidant defense system were detected in the lungs and alveolar type II (ATII) cells in emphysema patients (15, 17)

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