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We would also suggest the inclusion of a pediatric population

[DOI] [PMC free article] [PubMed] [Google Scholar] 496.Fernndez-Mendvil C, Luengo E, Trigo-Alonso P, Garca-Magro N, Negredo P, Lpez MG

doi: 10.1007/s00406-013-0413-4

Distribution of disaccharidase activity in the small bowel of normal and lactase-deficient subjects
The culture was split into two 300 ml cultures and each flask was diluted to 1.0 10 6 cells/mL, usually leading to a final volume of 600 mL that complies with the ratio of air to cell medium of 4:1
