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For the measurement of hypotaurine, taurine, and cystathionine, the prepared homogenate or medium was mixed with one volume of 5 % (wt/vol) sulfosalicylic acid, and the mixture was centrifuged at 15,000 g for 15 min at 4 C to obtain the acid supernatant

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This paper describes the first structure of a GPCRG-protein complex, highlighting receptor-mediated conformational changes within the G protein that are important for nucleotide release

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