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elegans Research Community), WormBook, doi/10.1895/wormbook.1.133.1

According to the recommendations provided by the European Association for the Study of the Liver (EASL), the following criteria are employed for the diagnosis of NAFLD: Exclusion of other causes of hepatic steatosis: The diagnosis of nonalcoholic steatohepatitis (NASH) necessitates the exclusion of alternative causes of liver fat accumulation, such as alcoholic liver disease, secondary steatosis (e.g., due to medication or genetic disorders), and hereditary hepatic steatosis

The stain was prepared by dissolving 0.5 g of Oil-Red-O in 100 mL of isopropanol, rocking for 3 days, diluting to 60% with milliQ-H 2 O, and filtering through a 0.45 m filter

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