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Both classes of enzymes are vulnerable to inactivation by electrophiles such as dehydro-alanine (acrylate) glutathione when they are in their reduced form

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[DOI] [PMC free article] [PubMed] [Google Scholar] 11.Wenz T, Rossi SG, Rotundo RL, Spiegelman BM, Moraes CT

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There is no single correct amount more water simply spreads the same 12 mg of peptide across a larger volume, which makes small doses easier to measure accurately