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Setoyama D, Lee HY, Moon JS, Tian J, Kang YE, Lee JH, et al
the derivatization reagent from the above step (1) is reacted with the test solution from above step (2) and the control solution from above step(3) respectively in the present of solvent at 20C 95C to give L-carnitine and D-carnitine derivatives;( 5 ) the content of L-carnitine and D-carnitine in test solution and control solution from the above step (4) is detected with HPLC, and the content of L-isomer L-3-cyano -2 - hydroxypropyl trimethyl ammonium and D-isomer D-3-cyano -2 - hydroxypropyl trimethyl ammonium is calculated
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