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The supernatant was removed, and the pellet was resuspended in 200 l of sonication buffer

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The mass spectrometer was operated in the positive electrospray ionization (ESI) mode, and the MS/MS analysis was performed in the multiple reaction monitoring (MRM) mode, monitoring the transitions of m/z 162.0 103.0 for carnitine, m/z 147.1 88.1 for IS, respectively

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A 2020 update did caution, however: Based on the currently available evidences, the role of ALC [acetyl-L-carnitine] in AD and other cognitive disorders is still under debate

RIP my friend