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Label-free protein quantification and bioinformatic analyses For differential ADP-ribosylome analysis, spectral counting-based quantification was utilized due to its efficiency in identifying the most robust proteomic changes during the initial proteomic screen

TMT labeling and fractionation Desalted peptides were labeled with TMTpro reagents (Thermo Fisher Scientific)

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Weeks 1324 (Plateau and Receptor Management): Extended cycles beyond 12 weeks continue to produce incremental fat loss and recovery benefits, but the rate slows as receptors adapt to chronic stimulation

Background GSS (Glutathione synthetase) is a 474 amino acid protein encoded by the gene located at human chromosome 20q11.2

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