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The remaining tobacco leaves were killed at 105 C for 30 min, dried at 75 C, and then ground to determine the nicotine content
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Cochleae were then dissected in PBS and blocked with 1% Triton X-100 and 5% normal horse serum in PBS for 1 h
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doi: 10.3389/fimmu.2021.628906 100 Di StasiADe AngelisBRooneyCMZhangLMahendravadaAFosterAEet al
