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c , Cdk4 knockdown increases forskolin-induced gluconeogenic gene expression (one-way ANOVA with Tukey post-test, n = 3)

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To further explore the in vivo effects of VGF suppression on glucagon secretion, we generated an AAV-pan vector expressing VGF shRNA, which has high transfection efficiency in the pancreas 23 , with non-targeting shRNA serving as a control (AAV-sh-Control) (Fig

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