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Lentiviral particles titers were determined by FACS analysis of GFP-positive cells

Panton-Valentine Leukocidin associated with S

& Tanksley, S

There is further evidence that HIV polyprotein precursors encoding these proteases are initially formed in an oxidized inactive state and need to be activated in a reducing environment 8,9,13,14,15 Moreover, the initial step in HIV-1 polyprotein processing, which is required to release the mature protease, is also regulated through reversible oxidation of cysteine 95 16

Another drug repurposed is metformin (clinically used as an anti-diabetic) which is being used as an AMPK activator, currently in phase II clinical trials for CLL (ClinicalTrials.gov: NCT01750567) (105, 106)

One-step purification of twin-strep-tagged proteins and their complexes on strep-tactin resin cross-linked with bis(sulfosuccinimidyl) suberate (BS3)
