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Unlike native IGF-1, this analog features a 13-amino acid N-terminal extension and an arginine substitution at position 3, conferring reduced affinity for insulin-like growth factor binding proteins (IGFBPs) and thus increased biological activity across multiple experimental models

Briefly, oocytes were treated with Tyrodes solution for 1 min to remove the zona pellucida

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The experiment was performed and data were analyzed by Wayen Biotechnologies (Shanghai, China)

Peh and Wong, 1999

SOPs allowed exclusion of ADEs based on lack of causality, which is non-compliant with FDA regulations requiring reporting regardless of relatedness