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Co-IP/pull down Cells were cultured in 10 cm dishes and lysed on ice for 30 min using ice-cold IP buffer (50 mM Tris-HCl, pH 7.5

IGF-1R is predominantly membrane-localized in benign glands, while malignant epithelium contains prominent internalized IGF-1R (located in the nucleus and cytoplasm)

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The intramuscular acidic saline injection model is another commonly used model that uses repeated injections of acidic saline into the gastrocnemius muscle to develop widespread pain 34,35

[URE3] as an altered URE2 protein: evidence for a prion analog in Saccharomyces cerevisiae