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Transient transfection, as above, was used to deliver DNA encoding mouse GLP-1RLgBiT and ARRB1/2-SmBiT fusion proteins

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Some studies indicate that aligning eating windows with circadian rhythms could affect hormone responses, but this area requires further investigation before specific recommendations can be made

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However, further inspection of the prohormone sequences revealed a second single basic amino acid followed by histidine residing 6 amino acids carboxyl-proximal to the first histidine, predicting a GLP-1 peptide of 31 amino acids, GLP-1(7-37)
