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SNAP/FLAG-hGLP-1R plasma membrane recycling INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto poly-L-lysine-coated black, clear bottom 96-well plates in full media, stimulated with vehicle or 100 nM exendin-4 for 1 hour at 37C to enable receptor internalisation, washed with PBS and incubated in 100 nM tetramethylrhodamine (TMR)-tagged exendin-4 (Ex-4-TMR) in full media for 1 hour or 3 hours at 37C to label any receptors that recycle back to the plasma membrane after agonist-mediated internalisation

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doi: 10.1152/ajpendo.00330.2023 21 LeeSHParkHYYunJHDoEK

An emerging role of dipeptidyl peptidase 4 (DPP4) beyond glucose control: potential implications in cardiovascular disease

The time-to-onset interval was subsequently computed as the temporal difference between EVENT_DT (the recorded date of adverse event manifestation) and START_DT (the documented date of therapeutic intervention initiation)