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In other experiments, acute stress also caused rapid downregulation of preproglucagon gene expression in the NTS neurons in rat brains [82]

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(91) found that the destruction of vagal afferent fibers by capsaicin or lesions to subdiaphragmatic vagal fibers abolished ghrelin-induced feeding and substantially decreased c-fos expression in the ARC, where vagal afferents terminate, in response to ghrelin in capsaicin-treated rats

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