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Comparing tirzepatide with B12 to other GLP-1 formulations Tirzepatide with B12 differs from semaglutide with B12 primarily through the dual GIP/GLP-1 mechanism

Quantitative real-time PCR was performed in 20 l of reaction mixture containing 1 l of cDNA, 10 pM of each primer ( Slc1a5 , Slc1a5 variant Gls1 , Gls2 , Cdkn2a , Cdkn1a , Cd38 , Igf1r , Il-1b , Il-6 and Il-10 ) and 10 l of SYBR Select Master Mix (Thermo Fisher Scientific) using Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific)

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Major insulin signaling pathways in the brain When insulin binds to its receptor, it activates two major intracellular signaling pathways, which regulate key brain functions, including neurotransmission, metabolism, energy balance, and neuronal survival [Figure 1]