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In brief, male mice were deeply anaesthetized with an intraperitoneal injection of 7% chloral hydrate and transcardially perfused with a modified ice-cold artificial cerebrospinal fluid (aCSF) (described below)

J Thorac Dis 7(5):920929 [DOI] [PMC free article] [PubMed] [Google Scholar] 10.Lyons MM, Bhatt NY, Pack AI, Magalang UJ (2020) Global burden of sleep-disordered breathing and its implications
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[DOI] [PubMed] [Google Scholar] 47.Rouse R, Xu L, Stewart S, Zhang J

Preliminary evidence suggests that a 12-Week treatment with tirzepatide plus low-energy ketogenic therapy is more effective than its combination with a low-calorie diet in preserving fat-free mass, muscle strength, and resting metabolic rate in patients with obesity
