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Acetylcholine acts on M3 muscarinic receptors expressed on endothelial cells, activating endothelial nitric oxide synthase (eNOS) and promoting NO production [20,21]
This phenomenon was initially explained by the loss of facial fat, which can lead to excess skin and profound wrinkles

Supportive care with cool compresses and over-the-counter antihistamines may provide relief if needed

In regards to the Necdin gene, which has been proposed as a potential candidate for PWS in humans due to its lack of expression in the brain, the mouse knockout model exhibited impaired development of GnRH neurons (217)

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