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Jans A, Nslund I, Ottosson J, Szabo E, Nslund E, Stenberg E

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After purification, islets were cultured overnight at 37 C in a humidified atmosphere of 95% air and 5% CO 2 in RPMI 1640 medium supplemented with 10% FBS, 100 units/ml penicillin and 0.1 mg/ml streptomycin

doi: 10.3389/fendo.2022.1004044 Received 26 July 2022 Accepted 27 September 2022 Published 13 October 2022 Volume 13 - 2022 Edited by ke Sjholm, Gvle Hospital, Sweden Reviewed by Finbarr P