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RNA isolation and gene expression Jejunal RNA was isolated with TRIzol Reagent (Ambion) as per the manufacturers protocol and reverse transcribed using a High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific 43-688-14)

Weight loss is a driver: rapid loss increases biliary stasis risk

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Efficacy and safety of luseogliflozin as monotherapy in Japanese patients with type 2 diabetes mellitus: a randomized, double-blind, placebo-controlled, phase 3 study