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Early increases in bile acids post Roux-en-Y gastric bypass are driven by insulin-sensitizing, secondary bile acids

Their ability to suppress appetite, delay gastric emptying, and enhance insulin sensitivity positions them uniquely to combat the multifaceted drivers of obesity

Tu Z, Yu Y, Li C, et al

Then incubated with the primary antibodies 1 in 500 dilution in 1% BSA and 0.25% triton 100 overnight at 4 C

Neuropharmacology (2014) 84:3145