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It hits GLP-1 and GIP receptors

Ghrelin levels rise, leptin signaling normalizes to pre-treatment levels, and the desire to eat increases substantially

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Monitor blood sugar closely, as remedies like methi amplify effects

Elevating cDNA synthesis reaction temperature to 50-55C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis
