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After 24 h incubation, fibroblasts were fixed with 4% (w/v) paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with QuickBlock Blocking Buffer (Beyotime, Shanghai, China), and incubated with ASC antibody (1:400, Cell Signaling Technology, Danvers, MA, USA, 67824s) at 4 overnight

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