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The low injection volume allowed precise loading of 1.8 mg/mL of each peptide in the hydrogel, and aliquots were collected over time for analysis via high-performance liquid chromatographymass spectrometry (HPLCMS) ( Figure 3A , Method S1 )

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Live birth rates were significantly lower in the class I and class IIIII obesity subgroups, and pregnancy rates were reduced across all body mass index groups, with statistical significance observed in the class I obesity subgroup, while findings in the overweight group were borderline and should be interpreted cautiously