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And so what can we do to make it easier

One concern exists

Roughly 12,000 beads were combined with the above ligation mix and incubated for 1 hour at 37C in an Eppendorf ThermoMixer (15 second alternative mixing at 1800 rpm)

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Cells were then washed with KRBH and imaged in 3 mM glucose KRBH with images taken every 12 seconds in a 60/1.4 NA oil immersion objective with a 561 nm laser in a Nikon Eclipse Ti microscope with an ORCA-Flash 4.0 camera (Hamamatsu) and Metamorph software (Molecular Devices)

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