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PC12-ND6 cells were seeded at a density of 20,000 cells per well in triplicate on poly-d-lysine-coated plates and treated with PBS (control) or NaBt (5 mM) for 24 h at 37 C in 5% CO2 atmosphere

Both male and female mice treated with intranasal GHK-Cu had significantly lower concentrations of NFL-1 in the frontal cortex compared to intranasal saline treated cohorts (Figure 4C) indicating GHK-Cu was able to attenuate axonal damage associated with brain aging in C57BL/6 mice