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For necroptosis induction, L929 cells (10,000 cells per well) were co-incubated with mouse TNF (10 ng ml 1 ), BV-6 (400 nM) and zVAD-FMK (30 M) for 24 h

Then the system was heated up from 200 K to 300 K in 20 ps with weak position restraints (10 kcal/mol 2 )

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This process is facilitated by a proton carrier in the inner mitochondrial membrane known as ATP synthase (also called complex V)