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Total proteins (30 g) were separated by SDS-PAGE and transferred onto a PVDF membrane (Millipore, Billerica, MA, USA)

It can also regulate apoptosis-related factors, reducing apoptosis and neural damage and reducing the amount of inflammatory cytokines and immunocyte stimulation [28,29]

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Effects of insulin and insulin-like growth factor I on growth of human leukemia cells in serum-free and protein-free medium