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For higher concentration studies, use 1.0 mL to achieve 5mg/mL, making 1mg equal 0.2 mL (20 units)

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Plasma TNF assay Blood samples were drawn from the dorsal vena cava, and plasma was obtained by centrifuging the blood at 8000 g for 15 min at 4 C

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Unfortunately, vitamin B12 deficiency can occur for several reasons