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Mobile phase A comprised water containing 0.1% formic acid and 5% acetonitrile and mobile phase B consisted of acetonitrile containing 0.1% formic acid and 5% water

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(a) Cohort in their 20s (n = 29)

The cytosolic GST enzymes comprise the largest family and based upon amino acid sequences and substrate specificities this family has been divided into seven subclasses identified as alpha (, A), mu (, M), omega (, O), pi (, P), sigma (, S), theta (, T), and zeta (, Z)
Medical therapy with hormones (hCG or LHRH), to stimulate testes descent and germ cell maturation is no longer suggested because of the lack of conclusive data

