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DOI: 10.1016/j.freeradbiomed.2005.07.002 71

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After washing once with PBS to remove unbound CFSE, SFN was added for 30 min and the cells were seeded on 96-well microplates coated with anti-CD3/CD28 antibodies for co-stimulation as described above

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Conventional and electronic cigarettes dysregulate the expression of iron transporters and detoxifying enzymes at the brain vascular endothelium: in vivo evidence of a gender-specific cellular response to chronic cigarette smoke exposure