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Let the vial sit undisturbed for 1-2 minutes

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The qRT-PCR, CCK8 assay, flow cytometry, Western blotting, immunofluorescence, hematoxylin and eosin, massons trichrome, immunohistochemical staining and transmission electron microscopy analysis were performed during in vivo and in vitro experiments

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The iron starvationlike response as the only significant genomewide consequence of GSH depletion did not befit ascribing a thiolredox control function at the origin of the GSH requirement for viability, which was previously established by the partial growth rescue of GSHdepleted cells by DTT (Sipos et al , 2002)