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Tail vein blood glucose concentrations were measured using a handheld glucometer (Contour XT, Bayers) at 0, 15, 30, 60 and 120 min after injection (for the Mc4r KO study a 90 min timepoint was also included)

The Dietary Guidelines for Americans recommend 25-30 grams of fiber daily for women and 30-38 grams for men, with attention to fiber type: Soluble fiber (oats, psyllium, apples, beans) forms a gel that softens stools Insoluble fiber (wheat bran, vegetables, whole grains) adds bulk and promotes transit Patients should increase fiber gradually over 2-3 weeks to minimize bloating

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p = 0.022), especially in patients at high CV risk (age 65 years, T2D duration 10 years and BMI 30 kg/m 2 ), but not the other DPP-4is, suggesting a differential effect of each DPP-4 inhibitor on the risk of HF [151]