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NanoBiT assay HEK 293T cells were seeded onto 6-well cell culture plates in DMEM containing 10% FBS and 1% sodium pyruvate at 37 C, 5% CO 2 before transfection

( A ) displays a bar graph quantifying normalized GLP-1R immunoreactivity in medium and small bronchi, with pancreatic tissue serving as a positive control and untreated tissue as a negative control

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The non-peptide GLP-1 receptor agonist WB4-24 blocks inflammatory nociception by stimulating -endorphin release from spinal microglia

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